Organic Syntheses, Coll. Vol. 7, p.530 (1990); Vol. 63, p.136 (1985).
Caution! A. Because of the hydrogen gas evolved during this reaction, this procedure should be carried out in an efficient fume hood.
An oven-dried, 3-L, three-necked flask
equipped with a mechanical stirrer
, a Friedrich condenser
, and a nitrogen-inlet tube
is flushed with nitrogen
, and then charged with a suspension of lithium aluminum hydride (47.9 g, 1.26 mol)
in 1200 mL of tetrahydrofuran (THF) (Note 1)
. The mixture is cooled (10°C, ice bath
) and L-valine (100 g, 0.85 mol)
is added in portions over a 30-min period from a 200-mL round-bottomed flask
connected to the reaction flask via a flexible plastic sleeve so as not to produce too vigorous an evolution of hydrogen (Note 2)
. After the addition is complete, the plastic sleeve is replaced by a stopper, the ice bath
is removed, and the reaction mixture is warmed to room temperature and then refluxed for 16 hr. The reaction mixture is then cooled again (10°C, ice bath
) and diluted with ethyl ether (1000 mL) (Note 3)
. The reaction is quenched over a 30-min period with water (47 mL) (Caution! See (Note 4)
, aqueous 15% sodium hydroxide (47 mL, over 20 min)
, and water (141 mL, over 30 min). The solution is stirred for 30 min and the white precipitate is filtered. The filter cake is washed with ethyl ether (3 × 150 mL)
and the organic filtrates are combined, dried with anhydrous sodium sulfate
, and concentrated under reduced pressure. Distillation of the residue under vacuum affords L-valinol
) (Note 5)
as a clear liquid: bp 63–65°C (0.9 mm) (Note 6)
: [α]D20 + 14.6°
1.455; IR (neat) cm1
: 3300, 1590; 1
H NMR (CDCl3
) δ: 0.92 (d, 6 H), 2.38–2.74 (m, 4 H), 3.13–3.78 (m, 2 H).
B. Caution! Because of the foul odor of the methyl sulfide given off, this procedure, up to the methanol quench, should be carried out in a hood.
A 2-L, three-necked, round-bottomed flask is equipped with a mechanical stirrer, heating mantel, 250-mL graduated addition funnel, and an 8-in., air-cooled reflux condenser (West type) topped with a water-cooled distillation head and a 1-L receiving flask
. It is connected to a nitrogen
line through the still head. The glassware is either oven-dried and cooled in a desiccator
or flame-dried and assembled while still hot. The assembly is flushed with nitrogen
and charged with 200 g of L-valine (1.7 mol)
, 400 mL of tetrahydrofuran (THF) (Note 1)
, and 210 mL of freshly distilled boron trifluoride etherate (242 g, 1.7 mol)
. The mixture is heated at a rate sufficient to cause the THF to reflux gently (Note 7)
and 188 mL (1.88 mol) of borane–methyl sulfide complex (BMS) (Note 8)
is added dropwise over the course of 2 hr (Note 9)
. The solution is then refluxed for 18 hr. The methyl sulfide
that has collected at the stillhead is discarded (Note 10)
, and the reaction mixture is cooled to 0°C and quenched by the slow addition of 200 mL of methanol
. The addition funnel is replaced by a glass stopper
, and the air-cooled condenser is removed, leaving the flask equipped for distillation of solvent through the distillation head. The reaction mixture is concentrated under reduced pressure with heating and stirring. The distillation head is replaced by a water-cooled reflux condenser
, and the residue is dissolved in 1 L of 6 M sodium hydroxide
and refluxed for 4 hr. The mixture is saturated with potassium carbonate (ca. 400 g)
; cooled; filtered through a Celite pad on a coarse, fritted funnel; and extracted with three 1-L portions of chloroform
. The combined extracts are washed with three portions of saturated sodium chloride (500 mL each)
, stirred over anhydrous potassium carbonate
for 24 hr, and concentrated under reduced pressure to give a yellow oil. The crude material is vacuum distilled to give 77.5 g
) of purified L
, bp 62–67°C/2.5 mm(Note 6)
; [α]D20 + 14.6°
1.455; IR (neat film) cm−1
: 3300 (OH), and 1590 (NH2
); NMR δ: 0.92 (d, 6 H), 1.54 (m, 1 H), 2.38–2.74 (m, 4 H), 3.13–3.78 (m, 2 H).
gas released during the addition of the amino acid should be vented through the nitrogen inlet to a bubbler at the back of the fume hood, well away from the mechanical stirrer motor and any other source of electrical spark.
Dilution is necessary to keep the reaction mixture from becoming too thick during the quench. Dilution with THF results in significantly lower yields.
An addition funnel was used for the dropwise addition of water. Care must
be taken during this quench to ensure that all the escaping hydrogen
gas is vented to the back of the fume hood through the nitrogen bubbler.
The submitters reported a yield of 73.7 g
The checkers found that L
) solidifies on distillation and will clog a water-cooled condenser. The use of a heat gun is recommended to avoid obstruction of the distillation pathway.
The temperature is maintained sufficiently high so that THF refluxes in the aircooled condenser while ether and methyl sulfide
distill through the short-path distillation head.
It is important that gentle reflux be maintained throughout the addition. If the solution is not heated during this period, an exothermic reaction occurs when the solution is refluxed.
should be destroyed by slowly pouring the volatile distillate into I gallon of household bleach (5% sodium hypochlorite
). After 30 min, the bleach solution may be discarded in the drain.
Procedure A using LAH is faster and more convenient than the BMS procedure (B). Both procedures are general for the reduction of amino acids to amino alcohols. If functional group incompatibility is precluded, lithium aluminum hydride reduction is preferable.
The borane procedure (B) is a hybrid of two methods, Lane's procedure for BMS
reduction of anthranilic acid
and Brown's procedure for enhanced-rate reductions of several functional groups with BMS by distilling off the methyl sulfide
during the course of the reaction.9
The submitters have obtained a 97% crude yield (44–51%
yield after distillation) of prolinol
using this procedure. Lane reports that the following additional amino acids can be reduced using BMS
, and 6-aminocaproic acid
Meyers has added phenylglycine
to the list, and has confirmed the optical purity of the amino alcohols obtained by preparation of the Mosher amides.10
This preparation is referenced from:
Copyright © 1921-, Organic Syntheses, Inc. All Rights Reserved